Abstract
CO2 laser pretreatment to improve the enzymatic hydrolysis of corn stover for production of monosaccharide, was investigated. Response surface methodology (RSM), at a three-variable, three-level experiment Box–Behnken design (BBD) established the following optimum pretreatment parameters: time, 67.53min; power, 264.33W; and liquid-to-solid ratio, 21.29:1 (mL/g). Under these conditions, the reducing sugars produced was 4.941mg/mL for cellulase hydrolysis. This amount matched the predicted value and increased cellulase hydrolysis from 14.47% to 30.84%. Scanning electron microscopy (SEM) showed that CO2 laser pretreatment converted the smooth into a rough and porous surface, which promoted the enzyme access and resulted in a high specific reducing sugars production rate.
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