Abstract
The present study is to explore the optimal extraction parameters, antioxidant activity, and antimicrobial activity of alkaline soluble polysaccharides from rhizome of Polygonatum odoratum. The optimal extraction parameters were determined as the following: NaOH concentration (A) 0.3 M, temperature (B) 80°C, ratio of NaOH to solid (C) 10-fold, and extraction time (D) 4 h, in which ratio of NaOH to solid was a key factor. The order of the factors was ratio of NaOH to solid (fold, C) > extraction temperature (°C, B) > NaOH concentration (M, A) > extraction time (h, D). The monosaccharide compositions of polysaccharides from P. odoratum were rhamnose, mannose, xylose, and arabinose with the molecular ratio of 31.78, 31.89, 11.11, and 1.00, respectively. The reducing power, the 1, 1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging rate, the hydroxyl radicals scavenging rate, and the inhibition rate to polyunsaturated fatty acid (PUFA) peroxidation of the alkaline soluble polysaccharides from P. odoratum at 1 mg/mL were 9.81%, 52.84%, 19.22%, and 19.42% of ascorbic acid at the same concentration, respectively. They also showed antimicrobial activity against pathogenic bacteria Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, and Escherichia coli.
Highlights
Polygonatum odoratum (Mill.) Druce is an edible herb mainly distributed in the Southern area of China and some other south east Asian countries [1], and it is found to be in Russia and other Europe countries [2, 3]
The objective of the work reported in this paper was to improve polysaccharides yield of alkaline soluble extraction from P. odoratum through an orthogonal test, which explored the relationship between four selected variables and determined the optimal variable values to optimize the alkaline extraction conditions, and to further investigate the bioactivity of the alkaline soluble polysaccharides extracts
The results indicated that alkaline soluble polysaccharides in P. odoratum were good DPPH scavengers
Summary
Polygonatum odoratum (Mill.) Druce is an edible herb mainly distributed in the Southern area of China and some other south east Asian countries [1], and it is found to be in Russia and other Europe countries [2, 3]. In particular in Hunan Province of southern China, cultivated P. odoratum is customarily called “Xiang Yuzu” by local people, and the dried rhizome is often used as a common Chinese traditional medicinal herb for removing dryness, promoting secretion, and quenching thirst [4]. It is used as functional foods and traditional Chinese medicines for treatment of diabetes [5]. The highest yield of hot water extraction assisted by microwave or ultrasound from P. odoratum was only nearly 7%, and three kinds of monosaccharides were detected, suggesting that there were plenty of polysaccharides in extraction residues insoluble in hot water. The objective of the work reported in this paper was to improve polysaccharides yield of alkaline soluble extraction from P. odoratum through an orthogonal test, which explored the relationship between four selected variables and determined the optimal variable values to optimize the alkaline extraction conditions, and to further investigate the bioactivity of the alkaline soluble polysaccharides extracts
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