Abstract
Pectinase enzyme finds extensive application in food and beverage industries. Pectinase production was studied in solid-state fermentation process using wheat bran and sugarcane bagasse as substrates utilizing Aspergillus niger . Optimization of media and fermentation conditions for maximum production of pectinase was carried out by one at a time procedure. Various combination of substrates were tried to achieve maximum pectinase production. The mixed substrates consisting of 90% of wheat bran and 10% of sugarcane bagasse gives maximum pectinase yield during the fermentation period of 96 h. The optimum temperature was found to be 40°C and optimum pH was found to be 5.The kinetics of pectinase production by solid state fermentation using Aspergillus niger was studied. The kinetic parameters value were found to be K m = 294.12 and V max = 2.33 h ml/U.
Highlights
Pectinolytic enzymes catalyzing the degradation of pectic substances are of great industrial importance (Alkorta et al, 1998)
The sugarcane bagasse appeared to be only inert support while growth of the microorganism and enzyme production stimulated by added wastes
The use of sugarcane bagasse as support is suitable for the growth of filamentous fungi and it allowed the utilization of high concentration of substrate solutions (Ooijkaas et al, 2000)
Summary
Pectinolytic enzymes catalyzing the degradation of pectic substances are of great industrial importance (Alkorta et al, 1998). Among processes used for enzyme production solidstate fermentation (SSF) is an attractive one because it presents higher productivity per reactor volume, lower capital and operating costs, lower space requirements, simpler equipment and easier downstream processing compared to that of submerged fermentation (SmF) (Acuna-Arguelles et al, 1995). It permits the use of agricultural and agro-industrial residues as substrates which are converted into bulk chemicals and fine products with high commercial value such as alcohol, organic acids, fats, proteins, enzymes, etc (Spagnuolo et al.,1998). Application of agro-industrial wastes as carbon sources in enzyme production reduces the cost of production and helps in solving disposal problems (Pandey et al, 2000)
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