Abstract

Achatina fulica was treated by pressurized hot water to extract bioactive polysaccharides and the extraction process was optimized with the extraction yield by response surface methodology (RSM). The optimized condition was temperature of 133.53 °C, water to solid ratio of 54.11 mL/g, and time of 14.95 min, and the yield of the polysaccharide was 42.39% ± 0.85%. The characterization of polysaccharide was comprised of preliminary, physical, and structural properties by GAG, uronic acid, and sulfate content, TLC, HPLC, GPC, FT-IR, UV–Vis spectroscopy, TGA, and XRD. The snail hydrolysis polysaccharides (SPs) was also demonstrated to its antioxidant by ABTS+ assay and total antioxidant activity of 32.30% ± 0.31% and 584.12 μg ± 2.10 μg ascorbic acid equivalent (AE)/L. This study indicated that pressurized hot water extraction can be used to extract polysaccharides from A. fulica.

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