Abstract
Seven morphologically distinct marine fungi were isolated from sediment and Seawater samples at different sites along Alexandria seashore. Antagonism effect against Aeromonas hydrophila on purpose and other pathogen was estimated. The most promising isolate giving the highest antibacterial activity (14 mm) against A. hydrophila was morphologically and genetically identified as Aspergillus terreus SHE05 and the corresponding sequence was recorded in the GenBank database with accession no. MW772239. Time course production of the antibacterial agents by A. terreus SHE05 against A. hydrophila was studied showing the highest productivity after 5 days incubation. Multi-factorial design in terms of Placket Burman design was implemented to predict the critical factors influencing the production of the antibacterial agents by A. terreus SHE05 against A. hydrophila. The obtained results showed that malt extract, pH and temperature were the key factors affecting the antimicrobial activity. Consequently, Box-Behnken design was applied to estimate the optimized levels of each independent variable showing that the optimized conditions were malt extract, 3 (g/l); peptone, 0.75 (g/l); salinity, 50%; pH, 4; culture age, 4 days; inoculum size, 0.5 ml; temperature 30°C and incubation time 5 days, which caused an increase in the antimicrobial activity to 25 mm, which denotes an approximately 1.8 fold increase comparing with the pre-optimized conditions. The potentiality of chloroform, hexane, petroleum ether and ethyl acetate for extraction of the active compounds was tested showing that ethyl acetate was the best. The extracted bioactive metabolites using ethyl acetate were tested as antimicrobial, anticancer, antiviral and antioxidant agents. Results showed reasonable activities. GC-MS was used to recognize the active components in the ethyl acetate extract, showing that the major compound was the Dodecanamine, N,N-Dimethyl with RT 11.95, molecular weight 213, area % (55.46) and molecular formula C14H31N.
Highlights
IntroductionThe management of diseases caused by bacteria is increasingly difficult due to the capability of bacteria to make resistance to antibacterial compounds. a critical need to achieve new groups of antagonistic agents exhibiting innovative mechanisms of action is essential. New developments in discovery of drugs from natural sources highlight on exploration of the marine environment to discover various novel and complex secondary metabolites for management of several diseases such as inflammatory condition, AIDS, cancer, malaria, fungal and viral diseases
The management of diseases caused by bacteria is increasingly difficult due to the capability of bacteria to make resistance to antibacterial compounds.1 a critical need to achieve new groups of antagonistic agents exhibiting innovative mechanisms of action is essential.2 New developments in discovery of drugs from natural sources highlight on exploration of the marine environment to discover various novel and complex secondary metabolites for management of several diseases such as inflammatory condition, AIDS, cancer, malaria, fungal and viral diseases.3Fungi produce a diversity of molecules with unique structures and display innumerable biological activities4-6 including anticancer, antiinflammatory, antiviral and antimicrobial
The highest antibacterial activity was against A. hydrophila with inhibition zone diameter (14 mm) followed by B. subtilis ATCC 6633 and E.coli ATCC 19404 recording inhibition zone diameters of 13 mm and 12 mm, respectively
Summary
The management of diseases caused by bacteria is increasingly difficult due to the capability of bacteria to make resistance to antibacterial compounds. a critical need to achieve new groups of antagonistic agents exhibiting innovative mechanisms of action is essential. New developments in discovery of drugs from natural sources highlight on exploration of the marine environment to discover various novel and complex secondary metabolites for management of several diseases such as inflammatory condition, AIDS, cancer, malaria, fungal and viral diseases.. New developments in discovery of drugs from natural sources highlight on exploration of the marine environment to discover various novel and complex secondary metabolites for management of several diseases such as inflammatory condition, AIDS, cancer, malaria, fungal and viral diseases.. Aspergillus is considered as the major source of fungal secondary metabolites and currently expanding its applications in different fields of pharmaceuticals and medicine.. Secondary bioactive metabolites production by microorganisms is influenced by the species and strains in addition to their cultural and nutritional conditions.. Response surface methodology (RSM) is a statistical tool, which is a valuable to enhance the factors affecting the processes of fermentation and was used in different optimization studies.. The purposefulness of the current research was assessment the potentiality of isolated marine fungus from the Mediterranean Sea as antimicrobial agents producer against the fish pathogen Aeromonas hydrophila.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
