Abstract
The objective of this research is to determinate the optimum conditions for the alcoholic fermentation process of aqueous jerivá pulp extract using the response surface methodology and simplex optimisation technique. The incomplete factorial design 3³ was applied with the yeast extract, NH4H2PO4 and yeast as the independent variables and the alcohol production yield as the response. The regression analysis indicated that the model is predictive, and the simplex optimisation generated a formulation containing 0.35 g L-1 yeast extract, 6.33 g L-1 yeast and 0.30 g L-1NH4H2PO4 for an optimum yield of 85.40% ethanol. To validate the predictive equation, the experiment was carried out in triplicate under optimum conditions, and an average yield of 87.15% was obtained. According to a t-test, no significant difference was observed (on the order of 5%) between the average value obtained and the value indicated by the simplex optimisation technique.
Highlights
Brazil is one of the most technologically advanced countries in terms of the production and use of ethanol as a fuel, positioning itself internationally as one of the largest producers and exporters of sugar cane and the largest producer and consumer of alcohol and as the only country to introduce the large-scale use of alcohol as an fuel alternative to petrol
Natural palm trees and their fruits could provide another resource for ethanol production because they contain the carbohydrates or sugars to enable alcoholic fermentation
The objective of this research was to study the influence of the following variables: yield, supplementation (NH4H2PO4) and yeast extract in the optimisation of ethanol production through the discontinuous alcoholic fermentation of aqueous jerivá pulp extract, combining the response surface methodology with a super-modified simplex method
Summary
Brazil is one of the most technologically advanced countries in terms of the production and use of ethanol as a fuel, positioning itself internationally as one of the largest producers and exporters of sugar cane and the largest producer and consumer of alcohol and as the only country to introduce the large-scale use of alcohol as an fuel alternative to petrol. As an alternative to ethanol production, researchers are seeking new raw materials with similar yields and viability to sugar cane Among these alternatives, natural palm trees and their fruits could provide another resource for ethanol production because they contain the carbohydrates or sugars to enable alcoholic fermentation. To increase the efficiency of finding the optimal factors, various statistical techniques have been proposed, factorial designs Among these techniques, response surface methodology (RSM) has been used by a significant number of researchers (AGUIRRE-GANZÁLES et al, 2011; BARBOSA et al, 2010; SILVA et al, 2008). The objective of this research was to study the influence of the following variables: yield, supplementation (NH4H2PO4) and yeast extract in the optimisation of ethanol production through the discontinuous alcoholic fermentation of aqueous jerivá pulp extract, combining the response surface methodology with a super-modified simplex method
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