Abstract
This research is based on the investigation of the bioremediation of diesel polluted soil using biostimulation strategy. The study was carried out on a diesel contaminated soil and concentrations: Tween 80 (5-15ml), Poultry droppings (20-60g), Hydrogen Peroxide (0.5-1.5ml) were added and incubated for 28 days of remediation period. Response Surface Methodology with Box-Behnken Design (BBD) was used with three factors and three levels of Tween 80, Poultry droppings and Hydrogen Peroxide as independent variables while diesel oil (Total Petroleum Hydrocarbon) removal was the dependent variable (response). The result showed disparities in the diesel oil biodegradation pattern with respect to Tween 80, Poultry droppings and Hydrogen Peroxide. The statistical analysis, via ANOVA showed coefficient of determination R2 to be 99.89% and P < 0.05. The predicted optimum parameter of Tween 80: 10.10ml, Poultry droppings: 41.46g and Hydrogen Peroxide: 1.10ml were gotten while 56.565% was gotten as the optimal diesel oil removal. At this optimum condition, the observed diesel oil removal was found to be 56.568%. It can be concluded that bioremediation of diesel-contaminated soil resulted in petroleum hydrocarbon degradation.
Highlights
Diesel is a fuel obtained from petroleum distillation and it is used in diesel engines
The experiment was designed using Response Surface Methodology, and after performing 17 experimental runs of the Box-Behnken design (BBD) and one control, the results of the statistical experiments were analyzed with reverence to the coded design matrix
The effect of different concentrations of hydrogen peroxide supplementation were investigated at the same condition of Poultry droppings and Tween 80 and the findings demonstrated that addition of hydrogen peroxide (H2O2) can enhance the bioremediation process of soil contaminated with diesel
Summary
Diesel is a fuel obtained from petroleum distillation and it is used in diesel engines. It is a lightweight mixture of liquid hydrocarbons and has a relatively low ignition temperature (540°C) and is ignited by the heat of compression. It is produced from the fractional distillation of crude oil between 200°C (392°F) and 350°C (662°F) at atmospheric pressure. When the soil becomes contaminated, the ecosystem is altered and agricultural activities are affected seriously due to this pollution. Physicochemical technologies have been previously used for the remediation of polluted soils [8]
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