Abstract
This study shows the study of optimal surface functionalization of nanoporous alumina membrane for "label-free" DNA detection. Single stranded DNA was first covalently immobilized on the nanopore walls via silane-PEG-NHS linker. The remained NHS group was hydrolyzed to form PEG layer to minimize the unspecific DNA binding during hybridization process. Optimal PEG-silane linker was achieved for better DNA immobilization efficiency. Using this optofluidic device, both ss-DNA immobilization and ds-DNA hybridization were successfully monitored via UV-Vis spectrum montoring. The nanopore size effect on DNA binding efficiency of membranes were also studied. With the increase of nanopore size, the DNA binding efficiency increased due to the increased reacted surface area. This portable optofluidic device integrated with nanoporos alumina membrane has the potential for nucleic acid in field detection in the application of food screening and environmental monitoring with high sensitivity
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