Optimal set of electrode potential enhances the toxicity response of biocathode to formaldehyde

Abstract

The autotrophic biocathode was promising as a broad spectrum, rapid-responding and sensitive sensing element for the early warning of toxicants in water. However, we found that the baseline current and the responsivity strongly relied on the cathode potential. Here we poised cathode potentials at 0, −0.2 and −0.4 V to investigate the effect of electrode potential on the sensor responsivity. With formaldehyde as the tested toxicant, the biocathode poised at −0.2 V had the highest baseline current (118.2 ± 10.7 A m−2) and the lowest toxicity response concentration (0.00148%), which exhibited a 6–64 times higher response ratio (1.4 × 104 A%−1 m−3) than those controlled at 0 V (2.3 × 103 A%−1 m−3) and −0.4 V (2.2 × 102 A%−1 m−3). First derivative of cyclic voltammetries revealed that the biocathode acclimated at −0.2 V had a highest main peak centered at 0.301 ± 0.006 V and several minor peaks between −0.2 to 0.2 V. Bacterial community analysis showed that Proteobacteria and Bacteroidetes families closely related to the sensing performance. Interestingly, Nitrospirae was obviously acclimated at −0.2 V, indicating that bacteria belonging to this phylum possibly contributed to the highest responsivity as well. Our findings revealed that the optimal set of electrode potential was critical to promote the toxicity responses of biocathode to the formaldehyde, and the differences were mainly from the microbial communities selected by different cathode potentials.