Abstract
Optical mapping of bacterial chromosomes provides an unambiguous low-resolution sequence scaffold of the entire chromosome. In comparison to some techniques, such as pulse field gel electrophoresis, cost and throughput limit the application of this technique outside of genome finishing. We have demonstrated the production of multiple bacterial maps using a single set of consumables; this significantly reduces the time and expense of map production.
Highlights
Optical mapping is increasingly used to characterize genomes [1,2,3]
Because DNA shears during the extraction process, optical mapping is not as simple as following a single chromosome; instead, fragments are assembled together in a process analogous to that used for genome assembly after sequencing
Maps can be used for finding large insertions, deletions, duplications and rearrangements otherwise difficult to characterize even with sequencing; and for scaffolding sequenced genomes without the use of numerous PCR reactions
Summary
Optical mapping is increasingly used to characterize genomes [1,2,3]. The map is an ordered restriction fragment list tracking the physical structure of each replicon without amplification. There are at least two ways to map multiple genomes on a single mapcard. This subdivides the surface area available for each DNA preparation but otherwise leaves the mapping process unaffected.
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