• Optical glucose biosensor with glucose oxidase was developed with a photonic interpenetrating polymer networks (IPN) structure. • Photonic IPN was prepared with solidified cholesteric liquid crystal (CLCsolid) and intertwined poly(2-dimethylaminoethyl methacrylate). • Glucose oxidase’s reaction with glucose redshifted the reflected color of the photonic IPN with high sensitivity and selectivity. • The developed glucose biosensor allowed naked-eye detection without any sophisticated analytical instruments. A patterned photonic array of dots with a photonic interpenetrating polymer network (IPN) structure consisting of intertwined photonic solid-state cholesteric liquid crystals (CLC solid ) and cationic polyelectrolyte networks was utilized for glucose detection. The photonic CLC solid network was prepared using a reactive mesogen mixture (RMM 727, Merck) doped with the chiral dopant CB15 1 1 (S)-4-cyano-4´-(2-methylbutyl)biphenyl (CB15) , followed by ultraviolet (UV) curing and chiral dopant extraction. The networked cationic polyelectrolyte was prepared by infiltrating a monomer mixture of DMAEMA 2 2 2-dimethylaminoethyl methacrylate (DMAEMA) and acrylic acid (AA) (85:15 mol%) and a crosslinker of TPGDA 3 3 Tripropylene glycol diacrylate (TPGDA) into the extracted space of the CLC solid network, UV curing under a photomask, washing the unreacted monomers outside the dots, and immobilizing the glucose oxidase (GOx) at the AA units via a coupling reaction with EDC 4 4 N-(3-dimethylaminopropyl)-N-ethylcarbodiimide (EDC) . The prepared patterned photonic dots display a clear pH response in terms of a redshift with decreasing pH because the intertwined poly(DMAEMA) (PDMAEMA) is a typical weak cationic polyelectrolyte which protonates and expands at pH values below its pK b . Thus, the enzymatic reaction of GOx with glucose causes a decrease in pH, expansion of the PDMAEMA, and a redshift of the reflected photonic color. The optimized photonic optical sensor shows a linear range of 0.7–12 mM with a detection limit of 0.021 mM, superior selectivity, and excellent spike test results with real blood serum. The developed photonic IPN sensor is attractive because it is cost-effective, battery-free, and allows easy naked-eye optical detection without the need for sophisticated analytical instrumentation.

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