Abstract
A monolithic column, prepared from a vinyl ester resin used as both monomer and cross-linking agent, has been used to remove matrix compounds from biological fluid. Using this column, nisoldipine in human plasma samples could be enriched online and the protein could be eluted. For this method of extraction, response (peak area) was a linear function of concentration in the range 2–80 ng mL−1, with a linear regression coefficient of 0.99985. The limit of detection for nisoldipine in plasma was 1.2 ng mL−1. For nisoldipine at concentrations of 10, 30, and 70 ng mL−1 RSD were 4.33, 6.63, and 4.57%, respectively. Recovery for both extraction (>73.6%) and the entire analytical method (>90.7%) were acceptable for screening of plasma for nisoldipine. The 12-hour pharmacokinetic profile of nisoldipine in mice after oral administration (0.3 mg kg−1) was investigated. The results indicated the method could be used for therapeutic monitoring of nisoldipine and enabled simple and rapid assay of the drug in plasma.
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