Abstract
A method for the trace level determination of carbofuran and 7-phenolcarbofuran in cervical-uterine epithelial tissue samples is proposed. The finely chopped tissue is methanol extracted in an ultra- sonic bath; the extract is evaporated, diluted with water and acidified to eliminate hydrophobic impurities by precipitation. Precon- centration and additional cleanup are carried out in a polymeric (PLRP-S) precolumn by loading the filtrated extract and successively washing with acetonitrile-water 5:95 (v/v) and 0.01 M aqueous ammonia. The precolumn is finally on-line eluted and analysed by reverse-phase HPLC with UV detection at 280 nm. Recoveries >90% and relative standard deviation of ∼12% were obtained in the analysis of non-contaminated tissue samples spiked at 0.2 μg/g of the pesti- cide and metabolite. Absolute limits of detection were 20 ng of ana- lyte in a cervical biopsy (150-950 mg of epithelial tissue). Application to the analysis of three samples from countrywomen exposed to carbofuran revealed the probable presence of 7-phenolcar- bofuran in two of them.
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