Abstract
We designed a microfluidic device to measure the trans-epithelial electrical resistance (TEER) and demonstrated that the tight junctions (TJs) of human renal proximal tubule epithelial cells (RPTECs) respond differently to the presence or absence of extracellular Ca2+ at the apical and basal sides. Once Ca2+ was removed from the basal side, the structure of TJs collapsed rapidly, whereas when the ion was removed from the apical side the collapse was gradual. After the removal of Ca2+, when it was resupplied to the basal side, the TJs recovered rapidly, whereas when the ion was replenished at the apical side, the recovery was gradual. We detected a subtle difference in the appearance of cell polarity. That indicates that our developed device is capable to accurately measure the TEER. The results of the deference in the response of TJs for extracellular Ca2+ may lead to the elucidation of the operating principle of the barrier function which tight TJs form.
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