OCIMUM AMERICANUM L. ESSENTIAL OIL EXHIBITS ANTIMICROBIAL ACTIVITY AGAINST ORAL BACTERIA RELATED TO PERIODONTAL DISEASE

Abstract

s. PHYTOPHARM 2012 M 106 Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] ТОМ 10/2012/2 ocimum americanum l. or hoary basil is an annual herbaceous plant native to Asia and Africa which has attracted increased interest due to its antimicrobial activity against a wide range of pathogenic microorganisms. To evaluate the antimicrobial property of essential oil extracted from the leaves of ocimum americanum against oral bacteria related to periodontal disease. Three species of periodontal pathogens including Porphyromonas gingivalis W50, Prevotella intermedia ATCC 25611 and Fusobacterium nucleatum ATCC 25586 were included in the study. Agar diffusion was performed initially to screen the antimicrobial activity of ocimum americanum essential oil. Minimum inhibitory concentration (MiC) and Minimum bactericidal concentration (MbC) were then determined using the Millipore membrane method. in the agar diffusion, essential oil extracted from ocimum americanum exhibited antimicrobial activity against all test bacteria with the zone of inhibition ranging from 24 to 30 mm. The MiC values against P. gingivalis and P. intermedia were 0.35 mg/ml whereas that of F. nucleatum was 0.70 mg/ml. The MbC values against P. gingivalis and P. intermedia were 0.70 mg/ml whereas that of F. nucleatum was 1.4 mg/ml. ocimum americanum essential oil has an antimicrobial activity which may be a beneficial component of oral health care products to control or prevent periodontal disease by reducing these bacteria in the oral cavity. OCIMUM AMERICANUM L. ESSENTIAL OIL EXHIBITS ANTIMICROBIAL ACTIVITY AGAINST ORAL BACTERIA RELATED TO PERIODONTAL DISEASE © Thaweboon Sroisiri, Thaweboon Boonyanit Faculty of Dentistry, Mahidol University, Bangkok, Thailand BREEDING AND SELECTION OF HIGH QUALITY PLANTS OF RHODIOLA ROSEA © Thomsen Mette Goul Norwegian Institute for Agricultural and Environmental Research (Bioforsk), Kapp, Norway The composition and content of chemical compounds are one of the most important factors when breeding medicinal plants. The chemical composition in R. rosea is mainly influenced by genetic factors but also factors like temperature and day length strongly affects the content of compounds. furthermore, clonal differences may exist in response to growth conditions and affect the concentration of metabolites. five Norwegian clones of R. rosea, two male and three female clones, from a clone collection was selected on the basis of the content of the marker compounds, total Rosavins and Salidrosid. The seeds from each of the female clones were collected and seed propagated plants from these three were planted at six different locations in Norway. After four seasons the plants were analysed for content of secondary metabolites. Content of Tot. Rosavines ( % of dry matter)