Abstract
Circoviruses, cycloviruses and other circular, replication-associated protein-encoding single stranded (CRESS) DNA viruses have been detected in a variety of animal taxa. In this study, cloacal swab samples (n = 90) were examined for CRESS DNA viruses from 31 wild bird species living at various aquatic sites in Hungary to identify possible reservoirs of viruses pathogenic to domestic poultry. A total of 30 (33.3%) specimens tested positive with pan-CRESS DNA virus specific PCR. Goose circovirus (GoCV), Duck associated cyclovirus 1 (DuACyV-1) and Garrulus glandarius associated circular virus 1 (GgaCV-1) were detected in nine, three and two different bird species, respectively. Selected specimens were subjected to whole genome sequencing. The obtained sequence data revealed conserved gene structure within the identified virus species and detected homologous (within GoCV) and possible heterologous recombination (within DuACyV-1) events. Results presented here provide new information on the genomic diversity and evolution of selected CRESS DNA viruses.
Highlights
Circoviruses, cycloviruses and other circular, replication-associated protein-encoding single stranded (CRESS) DNA viruses have been detected in a variety of animal taxa
Three groups of sequences were distinguished by direct sequencing and BLASTn search of the nested PCR products, showing similarities with the rep of Goose circovirus (GoCV, n = 9), Duck associated cyclovirus 1 (DuACyV-1, n = 17) and Garrulus glandarius associated circular virus 1 (GgaCV-1, n = 2) (Table 1)
In this report we studied the occurrence of CRESS DNA viruses in cloacal samples of wild birds
Summary
Circoviruses, cycloviruses and other circular, replication-associated protein-encoding single stranded (CRESS) DNA viruses have been detected in a variety of animal taxa. Goose circovirus (GoCV), Duck associated cyclovirus 1 (DuACyV-1) and Garrulus glandarius associated circular virus 1 (GgaCV-1) were detected in nine, three and two different bird species, respectively. The obtained sequence data revealed conserved gene structure within the identified virus species and detected homologous (within GoCV) and possible heterologous recombination (within DuACyV-1) events. A number of other circular, Rep-encoding single-stranded (CRESS) DNA viruses, which are currently not classified into these two genera, have been characterized and may differ considerably in the genomic structure and gene orientation; for example, the genes of these viruses are either unidirectionally or bidirectionally oriented and the encoded rep and cp can be partially overlapping[1,6,7,8]. Whole genome sequencing confirmed that the www.nature.com/scientificreports amplified gene fragments are parts of circular viral genomes and shed light on the evolutionary mechanisms of differentiation of the identified CRESS DNA viruses
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
