Occurrence and diversity of cultivable autochthonous microscopic fungi in substrates of old environmental loads from mining activities in Slovakia

Abstract

Distribution and biodiversity of soil microscopic fungi in 5 areas of old environmental loads in Slovakia were studied in relation to very low amount of organic matter (%TOC from 0.2 to 3.54) and to the pH gradient from ultra-acidic (< 3.5) to very strongly alkaline (> 9.0). All soil samples were affected by several hundred years of mining activities and contained heavy metals and other toxic elements: arsenic, cadmium, copper, zinc, antimony, lead. Concentrations of toxicants highly exceeded their limited values. Fifty-three genera and 112 species of microscopic fungi were identified. Among them, Zygomycota occurred very rarely (8 genera and 12 species), except of samples with the highest content of TOC (2.01–3.54% - samples 2 and 6), regardless their pH. Though, on the other hand, from some similar samples (3, 5 and 9), incl. those with relatively high TOC (0.14–2.62%), the lower fungi were not recovered. Forty one genera and 95 species of Ascomycota represented the most abundant fungal phylum in all investigated samples. Among them, Penicillium chrysogenum var. chrysogenum, Aspergillus niger and Neosartorya fischeri were isolated the most often. Phytopathogenic moulds of Bionectria ochroleuca, Lewia infectoria, Phoma macrostoma and Phlebia acerina were also occurred frequently. The highest biodiversity of microfungal community was recorded in the extreme acidic environment, followed by the neutral, ultra-acidic and the very strong acidic ones. There was no similarity in microfungal spectrum found in the samples studied. Except of the ultra acidic and extreme acidic samples (1−2) as well as the ultra acidic and strong acidic ones (1−4) with the most rich mycobiota, that may indicate a certain similarity degree.