Abstract
Ophiocordyceps sinensis has been used as one of the most valuable traditional Chinese Medication. This fungus parasitizes larva of Hepialus armoricanus, and converts each larva into a sclerotium form, in which the fruit body grows. Due to the geographical limitation, where O. sinensis can only be found in Himalayas region, the natural resources are limited and very expensive. This research aims to compare the growth-rate of O. sinensis mycelia with different ingredients mix with agar media using one-factor-at-a-time method. This research demonstrated the mycelial growth-rate with different carbon sources, including monosaccharide (Fructose, Glucose), disaccharide (Maltose, Sucrose), and polysaccharide (Dextrin, Malt extract), complex organic nitrogen sources, including beef extract, yeast extract, whey protein, and soy protein, and eight different carbon to nitrogen ratios. The objective of this research is to find out the suitable carbon and organic complex nitrogen sources and ratio for the O. sinensis solid cultivation. As results, O. sinensis grew best with disaccharides comparing to the other types of carbon sources. Furthermore, O. sinensis preferred whey protein in contrast to other organic complex nitrogen sources. As for the carbon to nitrogen ratios, an optimal ratio of 18:1 was observed. Based on those experiments, carbon source shows a greater influence for the mycelial growth. Hence many different types of grains and cereals would be great candidates as the main ingredients for the O. sinensis solid cultivation.
Highlights
IntroductionOphiocordyceps sinensis has been used as one of the most valuable traditional
This research aims to compare the growth-rate of O. sinensis mycelia with different ingredients mix with agar media using one-factor-at-a-time method
This research demonstrated the mycelial growth-rate with different carbon sources, including monosaccharide (Fructose, Glucose), disaccharide (Maltose, Sucrose), and polysaccharide (Dextrin, Malt extract), complex organic nitrogen sources, including beef extract, yeast extract, whey protein, and soy protein, and eight different carbon to nitrogen ratios
Summary
Ophiocordyceps sinensis has been used as one of the most valuable traditional. In 2015, O. sinensis cost as much as 60,000 U.S dollar per kilogram [1]. O. sinensis has a unique way of life and earns this caterpillar fungus a name in Chinese called “Dong Chong Xia Cao”, which roughly describes the complexity of the fungus’ life cycle [2]. The life cycle of O. sinensis includes an asexual and a sexual phase. This fungus parasitizes larva of Hepialus armoricanus, and converts each larva into a sclerotium, where the stroma and fruiting body may form under appropriate conditions [3] [4] [5] [6]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
