Abstract

The recent works have shown the unicellular green alga Chlamydomonas reinhardtii is a relevant model for investigations of algal bioprocesses. In the current work, several media were evaluated in batch mode for a better understanding of C. reinhardtii metabolism. Nutrient-suppression using heterotrophic and mixotrophic conditions were performed. The findings showed C. reinhardtii metabolized lactose (from milk whey permeate) resulting in high biomass density (2.08g/L) and total chlorophyll content (86.74mg/m3). It was observed a specific growth rate of 0.023h and 29h for the doubling time. In sulfur-suppression, the algal growth (1.17g/L) was reduced even though a carbon source (glucose) has been supplemented. Also, the specific growth rate (0.022h) and the doubling time (31h) was verified. The production of ethanol was slight and the acetic acid-suppression affected the C. reinhardtii performance providing slow cell growth (0.004h) and high doubling time (154h).

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