Abstract

An experimental apparatus for the analysis of biological cells light scattering in liquid suspensions has been presented. Characterization is based on the scattering of a monochromatic laser beam by particles [which can be inorganic, organic, or biological (such as animal cells and bacteria)] and on the strong relation between the light-scattering pattern and the morphology and refractive index of the particles. In order to study light scattering in biological cells close to the actual situation, we focus on non-spherical particles in the cell-culture medium. Finally, we demonstrate the light scattering results of bovine kidney cells suspended in the cell-culture medium, and compares then with the simulated results.

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