Abstract
Temperate Bacillus subtilis phage SPO2 codes for a phage-specific DNA polymerase. The polymerase gene has been cloned, and its nucleotide sequence has been determined. Within the sequence there is an open reading frame starting with a TTG and ending with three consecutive translational stop codons. Ten base pairs upstream from the proposed TTG initiation codon there is a probable ribosome-binding site with a calculated free energy of interaction with the 3' end of B. subtilis 16S rRNA of -15 kcal (-63 kJ)/mol. Based on the sequence and the expression of the polymerase gene in three different hybrid plasmids, we conclude that this open reading frame is the structural gene for SPO2 DNA polymerase. The predicted molecular weight of the polymerase is 72,486. In hybrid plasmid pJB74, the terminal triplet of an open reading frame with coding capacity for a protein of ca. 10 kilodaltons overlaps with the translational initiation triplet TTG of the polymerase gene. We speculate that transcription and translation of this open reading frame can influence the amount of phage DNA polymerase made in SPO2-infected bacteria.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
