Abstract
Two Shiga toxin (Stx) genes (stx-1 and stx-2) were cloned and sequenced from a strain of enterohemorrhagic Escherichia coli, serotype O157:H7, a clinical isolate from the Gifu City, Japan, outbreak in 1996 (GPU96MM). The 1,257 bp of stx-1 and 1,321 bp of stx-2 genes were analyzed, and each gene contained two open reading frames (ORFs), 948 or 960 bp of A and 270 bp of B subunits, respectively. Although the nucleotide sequences of stx-1 ORFs of this isolate had several nucleotide alterations, the deduced amino acid sequence was identical to that of stx-1 from E. coli (O157:H7) isolated from Canada and stx from Shigella dysenteriae type 1. The nucleotide sequences of the stx-2 ORFs of this strain were identical to those of stx-2 from two E. coli (0157:H7) strains, KNIH317 isolated from Korea and 933.
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