Abstract
The samples of cerebrospinal fluids from patients with ischemic stroke and motor neuron disease and from persons with no neurological diseases were studied by 1H NMR spectroscopy. Conditions for cerebrospinal fluid analysis were optimized. Twenty-five low-molecular-weight resident metabolites were identified and quantitatively determined in the cerebrospinal fluid. It was found that the concentrations of lactate, citrate, glucose, creatine, alanine, acetate, pyruvate, acetoacetate, formate, 2-hydroxybutyrate, 3-hydroxybutyrate, tyrosine, phenylalanine, valine, 2-hydroxyisovalerate, glutamine, fructose, isoleucine, and mannose in patients with ischemic stroke were several times higher than those in patients from the control group. Differences between the compositions of cerebrospinal fluid in the control group and a group of patients with motor neuron disease were detected with respect to the concentrations of 3-hydroxybutyrate, histidine, acetate, and mannose. The statistical processing of cerebrospinal fluid analysis data with the use of the method of principal components makes it possible to distinguish among the groups of cerebrospinal fluid samples from patients with ischemic stroke and motor neuron disease and persons from the control group. The results and the proposed approach to obtaining data can form the basis of the development of diagnostic models.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
