İnsan Kanser Hücrelerinde Astragalus elongatus subsp. nucleiferus’ un Antikanser, Antiproliferatif ve Laktat Dehidrojenaz Enzim Aktiviteleri

Abstract

In this study, potential anticancer and antiproliferative activities of ethanol and water extracts from aerial parts and roots of Astragallus elongatus subsp. nucleiferus were evaluated. In this context, MTT, trypan blue, and LDH enzyme activity assays were performed to determine cytotoxicity and cell proliferation potentials of the plant extracts against human A549, H1299, C6 cancer cells, and non-tumorous HUVECs. Based on cytotoxicity against the cancer cells, the various concentrations of the extracts were selected for in vitro assays. As a result of MTT and trypan blue assays, dose-dependent anticancer and antiproliferative effects were observed on tested human lung and brain carcinoma cells. The water extracts obtained from root of the plant exerted higher anticancer activity with IC50 values ranging from 5.81±0.46 to 18.24±0.12µg/mL than the aerial parts of the extracts. Besides increasing extract concentration lead to decrease the growth rate of the cancer cells, apoptosis was observed in the tested human malignant cells. In contrary anticancer and cytotoxicity results, the ethanol extract of the root was also caused to the highest apoptosis level in a dose dependently. Regarding LDH activity, the plant extracts obtained from aerial parts and roots were demonstrated the highest LDH activity towards H1299 cells, which was followed by A549 cells. The results were found a similar activity profile as observed in MTT and trypan blue assays. These results obtained from this work showed that the roots of the plant are able to inhibit cell growth in cancer cells in a time and concentration dependently.