Abstract
Papillomaviruses (PVs) are associated with the development of neoplasias and have been found in several different species, most of them in humans and other mammals. We identified, cloned and sequenced PV DNA from pigmented papilloma-like lesions of a diamond python (Morelia spilota spilota). This represents the first complete PV genome discovered in a Squamata host (MsPV1). It consists of 7048 nt and contains the characteristic open reading (ORF) frames E6, E7, E1, E2, L1 and L2. The L1 ORF sequence showed the highest percentage of sequence identities to human PV5 (57.9%) and Caribbean manatee (Trichechus manatus) PV1 (55.4%), thus, establishing a new clade. According to phylogenetic analysis, the MsPV1 genome clusters with PVs of mammalian rather than sauropsid hosts.
Highlights
Papillomaviruses (PVs) are associated with the development of neoplasias and have been found in several different species, most of them in humans and other mammals
We report on the cloning, sequence determination and phylogenetic analysis of a PV-specific DNA from the Australian diamond python (Morelia spilota spilota)
The coding sequences for the E1, E2, L2 and L1 proteins from those fifty papillomaviruses including MsPV1 and the 5 previously published sauropsoid PVs were aligned on the amino acid level by using the Perl script transAlign.pl [14]
Summary
Papillomaviruses (PVs) are associated with the development of neoplasias and have been found in several different species, most of them in humans and other mammals. The family may be divided into at least 29 genera with a vast number of species, types, subtypes, and variants of papillomaviruses (PVs), based on nucleotide identities of the L1 open reading frames (ORFs) [2]. DNA of PVs can be detected in a wide range of vertebrate species, far including humans, various other mammals, birds, and turtles (additional file 1).
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