Abstract
A novel positive-sense, single-stranded RNA (+ssRNA) virus (Halastavi árva RNA virus, HalV; JN000306) with di-cistronic genome organization was serendipitously identified in intestinal contents of freshwater carps (Cyprinus carpio) fished by line-fishing from fishpond “Lőrinte halastó” located in Veszprém County, Hungary. The complete nucleotide (nt) sequence of the genomic RNA is 9565 nt in length and contains two long - non-in-frame - open reading frames (ORFs), which are separated by an intergenic region. The ORF1 (replicase) is preceded by an untranslated sequence of 827 nt, while an untranslated region of 139 nt follows the ORF2 (capsid proteins). The deduced amino acid (aa) sequences of the ORFs showed only low (less than 32%) and partial similarity to the non-structural (2C-like helicase, 3C-like cystein protease and 3D-like RNA dependent RNA polymerase) and structural proteins (VP2/VP4/VP3) of virus families in Picornavirales especially to members of the viruses with dicistronic genome. Halastavi árva RNA virus is present in intestinal contents of omnivorous freshwater carps but the origin and the host species of this virus remains unknown. The unique viral sequence and the actual position indicate that Halastavi árva RNA virus seems to be the first member of a new di-cistronic ssRNA virus. Further studies are required to investigate the specific host species (and spectrum), ecology and role of Halastavi árva RNA virus in the nature.
Highlights
Picorna-like viruses are a loosely defined group of nonenveloped, positive-sense single-stranded RNA (+ssRNA) viruses that are major pathogens of humans, animals, insects and plants [1]
ORF2 is followed by a 39UTR of 139 nt
No large open reading frames (ORFs) were found in the inverse orientation suggesting that Halastavi arva RNA virus is a positive-strand RNA virus with dicistronic genome organisation
Summary
Picorna-like viruses are a loosely defined group of nonenveloped, positive-sense single-stranded RNA (+ssRNA) viruses that are major pathogens of humans, animals, insects and plants [1]. These viruses have similar genome features and several conserved protein domains. Iflaviruses, marnaviruses, picornaviruses and secoviruses have monocistronic genome structure with a large open reading frame (ORF) coding a single polyprotein. Dicistroviruses - the name (dicistro) is derived from the characteristic di-cistronic arrangements of the genome - have two non-overlapping large open reading frames (ORF1 and ORF2). The structural proteins are located at the 39 end (ORF2) rather than the 59 end as found in iflaviruses, marnaviruses, picornaviruses and secoviruses. It is proposed that dicistrovirus Plautia stali intestine virus (PSIV) ORF1 polyprotein precursor contains all proteins (2A–C, 3A–D) and in a same order as in picornaviruses [4]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
