Abstract
To assess genetic variation, 33 novel microsatellite markers were identified through nucleotide sequencing of enriched genomic libraries of Pteris cretica. Di- repeats (79.7 %) were found to be most predominant followed by tri (15.8 %), tetra (2.3 %) and hexa (2.3 %) type of repeat motifs. Evaluating these markers in six populations (N = 48) of Western Himalayan range detected average polymorphism information content (PIC) of 0.32. Combined neighbor joining (NJ) and principal coordinate analysis (PCoA) grouped all the populations in two major clusters with high levels of intermixing of accessions in each cluster. This suggests that P. cretica populations of Western Himalaya have broadly been mixed with two sub-populations. High within population variance (98.7 %) and low genetic differentiation (Φst: 0.013), recorded in the analysis of molecular variance (AMOVA). For the first time, highly polymorphic novel genomic microsatellite markers were identified and utilized for revealing genetic diversity of P. cretica in Western Himalayan range in context of established hypothesis of genetic variations based on allozyme markers.
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