Abstract
Bentonite exfoliation liberated the interlayer surface with large area for immobilization of enzyme at high enzyme loading. Upon the electrostatic attraction between positively charged bovine pancreatic lipase (BPL), Yarrawia lipolytica lipase (YLL), trypsin and negatively charged surface of unilaminar bentonite, three nanohybrids with a stably card-like structure named as BPL-B, YLL-B and TB were facilely produced. These nanohybrids showed higher activities in hydrolysis as 115.2% (BPL-B), 154.8% (YLL-B) and 138.2% (TB) of that of the correspondingly free enzyme. Furthermore, BPL-B, YLL-B and TB retained 80%, 90% and 82% of each original activity after repeated use for 10, 12 and 8 times, respectively. The recyclable and active nanohybrids are expected to be widely applied as industrial catalysts in the future.
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