Abstract
Three-dimensional (3D) cells in vitro culture are becoming increasingly popular in cancer research because some important signals are lost when cells are cultured in a two-dimensional (2D) substrate. In this work, bacterial cellulose (BC)/gelatin hydrogels were successfully synthesized and were investigated as scaffolds for cancer cells in vitro culture to simulate tumor microenvironment. Their properties and ability to support normal growth of cancer cells were evaluated. In particular, the human breast cancer cell line (MDA-MD-231) was seeded into BC/gelatin scaffolds to investigate their potential in 3D cell in vitro culture. MTT proliferation assay, scanning electron microscopy, hematoxylin and eosin staining and immunofluorescence were used to determine cell proliferation, morphology, adhesion, infiltration, and receptor expression. The in vitro MDA-MD-231 cell culture results demonstrated that cells cultured on the BC/gelatin scaffolds had significant adhesion, proliferation, ingrowth and differentiation. More importantly, MDA-MD-231 cells cultured in BC/gelatin scaffolds retained triple-negative receptor expression, demonstrating that BC/gelatin scaffolds could be used as ideal in vitro culture scaffolds for tumor cells.
Highlights
There are over 200 different types of cancer in the world
Proliferation and differentiation inside after short duration. These results after short duration culture. These results suggested that modification of with gelatin could be suggested that modification of bacterial cellulose (BC) with gelatin could be conducive to proliferation, adhesion, conducive to proliferation, adhesion, differentiation and ingrowth of cells.hydrogel
Gelatin was introduced into BC scaffolds and wrapped the pure BC nanofibers
Summary
There are over 200 different types of cancer in the world. It is predicted that one in three people will develop some form of cancer in their lifetime. In a study by Szot and colleagues [11], electrospun polycaprolactone (PCL)/collagen I scaffold was investigated as a potential 3D scaffold for an in vitro cancer model. Due to its excellent properties, including remarkable mechanical properties in both dry and wet states, high moldability, high water-holding capacity and high porosity, BC has been investigated as scaffolding in tissue engineering studies, with results demonstrating suitable cell proliferation, differentiation and adhesion [16,17,18]. The potential and biological activity of BC/gelatin for tissue engineering applications and in vitro 3D culture have not yet been studied systematically. Investigation of the cancer cellular responses to the scaffolds to evaluate BC/gelatin used cancer cell in vitro 3D culture
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