Abstract
Nothotylenchus andrassy n. sp. is described and illustrated from moss ( Sphagnum sp.) based on morphology and molecular analyses. Morphologically, this new species is characterized by a medium body size, six incisures in the lateral fields, and a delicate stylet (8-9 µm long) with clearly defined knobs. Pharynx with fusiform, valveless, non-muscular and sometimes indistinct median bulb. Basal pharyngeal bulb elongated and offset from the intestine; a long post-vulval uterine sac (55% of vulva to anus distance); and elongate, conical tail with pointed tip. Nothotylenchus andrassy n. sp. is morphologically similar to five known species of the genus, namely Nothotylenchus geraerti , Nothotylenchus medians , Nothotylenchus affinis , Nothotylenchus buckleyi , and Nothotylenchus persicus . The results of molecular analysis of rRNA gene sequences, including the D2-D3 expansion region of 28S rRNA, internal transcribed spacer (ITS) rRNA and partial 18S rRNA gene are provide for the new species.
Highlights
The genus Nothotylenchus Thorne, 1941 belongs to subfamily Anguinidae Nicoll, 1935 within the family Anguinidae Nicoll, 1935. Dong-Geun et al (2005) showed that the type species of the genus, Nothotylenchus acris, is a parasite of strawberry (Fragaria × ananassa Duchesne). Nishizawa and Iyatomi (1955) reported this nematode in association with strawberry diseases in Japan
Nothotylenchus species are morphologically closely related to members of Ditylenchus Filipjev, 1936, but they differ in morphology of the median pharyngeal bulb which is indistinct or non-muscular, non-valvate in Nothotylenchus
This publication includes a description of Nothotylenchus andrassy n. sp. through morphological observation and molecular characterization by the partial 18S rRNA, D2–D3 expansion region of 28S rRNA and internal transcribed spacer (ITS) rRNA gene sequences
Summary
The genus Nothotylenchus Thorne, 1941 belongs to subfamily Anguinidae Nicoll, 1935 within the family Anguinidae Nicoll, 1935. Dong-Geun et al (2005) showed that the type species of the genus, Nothotylenchus acris, is a parasite of strawberry (Fragaria × ananassa Duchesne). Nishizawa and Iyatomi (1955) reported this nematode in association with strawberry diseases in Japan. Relationships within Anguinidae were not well resolved in the phylogenetic analysis using the D2–D3 expansion segments of 28S, ITS, and partial 18S rRNA gene sequences (Subbotin et al, 2004). Through morphological observation and molecular characterization by the partial 18S rRNA, D2–D3 expansion region of 28S rRNA and ITS rRNA gene sequences.
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