Abstract
The use of polyethylenglycol (PEG) as an agent for protoplast fusion of different organisms, including animal cells and fungi, has been known for over 20 years. However, it was not before 1976 that a successful fusion of bacterial protoplasts with PEG was performed with polyauxotrophic strains of B. subtilis (Schaeffer et al., 1976) and with B. megateriitm (Fodor and Alfbldi, 1976). The basic information and progress on fusion of bacterial protoplasts since then has been reviewed at this colloquium by R. Hotchkiss. Therefore, the communications and discussions at that workshop were focused mostly on one class of B. subtilis PEG induced fused products (Hotchkiss and Gabor, 1980). This class of diploids, named initially “biparental”, are non-complementing diploids (Ned). The strains carry the two parental chromosomes, but only one of the two is expressed. The other one is phenotypically “silent”. Segregation of Ned clones into the parental types is usually 10-2 to 10-4
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