Abstract
Presented is a comprehensive approach to utilization of the wastes that appear in the process of heterologous anti-rabies immunoglobulin production (packed red cells, fibrin, and alcohol-containing products). Specific immunoglobulin is extracted from the surface of red blood cells using desorption technique. Additional yields of immunoglobulin after exposure of erythrocytes to non-ionic detergent amount to 10-19 % of the output. Rich protein supplement feeding for horses-producers is obtained from spray-dried packed red cells. Solid nutritious substrate for microbiological media production is obtained from fibrin using enzymic hydrolysis method. The efficiency of the fibrin hydrolysate-based media is 1.5-2 times higher in comparison with that of the media based on the digest of meat and casein, as demonstrated by the results of Vibrio cholerae scaled cultivation. Furthermore, worked out is the technology of ethanol regeneration after the rivanol-ethanolic precipitation of gamma globulin, alcohol content by volume being (93±1) % after the regeneration. It is demonstrated that the regenerated alcohol can be used as a precipitator in the process of anti-rabies serum fractioning. All in all, the developed techniques make it possible to utilize the wastes of anti-rabies immunoglobulin production and provide for further use of derivatives while producing medical immunobiological preparations.
Highlights
Presented is a comprehensive approach to utilization of the wastes that appear in the process of heterologous anti-rabies immunoglobulin production
Specific immunoglobulin is extracted from the surface of red blood cells using desorption technique
Solid nutritious substrate for microbiological media production is obtained from fibrin using enzymic hydrolysis method
Summary
В работе использовали отходы производства гетерологичного АИГ – фибрин и эритроцитарную массу из гипериммунной сыворотки крови лошадейпродуцентов, а также спиртосодержащий сток после риванол-спиртового осаждения гамма-глобулина. Гидролизат фибрина для использования в качестве основы питательных сред готовили методом ферментации фаршем из поджелудочной железы КРС [12]. Уровень специфической активности десорбированного АИГ и иммуноглобулина, выделенного с использованием регенерированного этилового спирта, определяли двумя способами: in vivo – биологическим методом в реакции нейтрализации вируса бешенства на белых мышах [15], и in vitro – в дотиммуноанализе [14]. Спиртосодержащий отход (фугат) собирали в накопитель на конечном этапе выделения гамма-гло булина после прохождения смеси фильтрата антирабической сыворотки и 25 % этилового спирта через сверхцентрифугу СГО 100 и перерабатывали на сконструированной оригинальной регенерационной установке. Определение концентрации этилового спирта проводили ареометрическим методом на ареометре АСп-3 с погрешностью спирта по объему 0,5 %
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