Non symbiotic germination and growth of the orchid seeds. I

Abstract

Experiments were conducted to determine the most favorable medium for germination and growth of seedlings on sterilized culture of Cymbidium, The results obtained are as follows:1. KNUDSON′S C solution or BURGEFF′S Eg-1 solution gives better growth than both KNUDSON′S B solution and tomato juice medium for non-symbiotic germination.2. Fish soluble medium with pepton added accelerates the growth of protocorms and seedlings of Cymbidium.3. The growth of embryos is enhanced in existence of KNUDSON′S C solution containing L-alginine or L-asparatic acid but not containing glutamic acid. Of the vitamins used as the additive, riboflavin and ascorbic acid also promote their growth.4. Potassium salt of l-naphthaleneacetic acid (NAA) in the medium inhibits the germination of seed and growth of protocorms in a short term culture. However, the injection of NAA solution on the medium after the germination resulted in an promotive effect of growth of seedlings. It seems that NAA affects more inhibitory on germination than on later development. It is elucidated by the fact that if NAA solution of lower concentration (0.1-1.0ppm) is given in the medium, it promotes the growth of seedlings in the later stage of growth. Especially in the transplanting medium NAA has a good effect on the growth of seedlings.5. Extract of silkworm-pupa as the additive stimulate, but extract of globule powder has no effect on the growth of protocorm and seedlings.