Abstract
Mitochondrial function has been predominantly measured ex vivo. Due to isolation and preservation procedures ex vivo measurements might misrepresent in vivo mitochondrial conditions. Direct measurement of in vivo mitochondrial oxygen tension (mitoPO2) and oxygen disappearance rate (ODR) with the protoporphyrin IX‐triplet state lifetime technique (PpIX-TSLT) might increase our understanding of mitochondrial dysfunction in the pathophysiology of acute disease.LPS administration decreased mitochondrial respiration (ODR) in vivo but did not alter mitochondrial function as assessed with ex vivo techniques (high resolution respirometry and specific complex determinations). PpIX-TSLT measures in vivo mitoPO2 and ODR and can be applied non-invasively at the skin.
Highlights
Acute changes in mitochondrial function may play a role in the pathophysiology of sepsis (Fink, 2002)
MitoPO2 measurements In accordance with previous experiments (Harms et al, 2015a), the application of 5-Aminolevulinic acid (ALA) increased the amount of Protoporphyrin IX (PpIX) in the mitochondria to detectable levels in all animals
The major findings of this study are that in vivo respirometry and ex vivo respirometry provide different results in our endotoxemic rat model and that PpIX-TSLT provides a sensitive means to measure aspects of mitochondrial function in vivo
Summary
Acute changes in mitochondrial function may play a role in the pathophysiology of sepsis (Fink, 2002). The options for monitoring mitochondrial function in patients are limited since only ex vivo techniques are available. Respirometry measures oxygen consumption in suspensions of isolated cells, isolated mitochondria or homogenates of small tissue biopsies and, might not adequately reflect the in vivo situation in acute changes of mitochondrial function (Jeger et al, 2013). NMR and NADH fluoroscopy both show determinants of metabolic state. Changes of mitochondrial metabolic states measured by NADH fluoroscopy have been shown in a research setting. Despite these results, standard clinical monitoring of mitochondrial function by NADH fluoroscopy is not yet an option due to its sensitivity to artifacts and difficult interpretation
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