Nocardioides xinjiangensis sp. nov., a novel species isolated from desert soil.

Abstract

Two novel strains, SYSU D00514T and SYSU D00778, were isolated from desert soil in the Gurbantunggut Desert, Xinjiang Uygur Autonomous Regions, PR China. SYSU D00514T and SYSU D00778 were aerobic, Gram-stain-positive, rod-shaped, catalase-positive and oxidase-negative. SYSU D00514T grew at temperatures ranging from 4 to 37°C (optimum, 28-30°C), at pH 6.0-8.0 (optimum, 7.0), and tolerated NaCl concentrations from 0 to 7.5% (optimum, 0.5% w/v). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains SYSU D00514T and SYSU D00778 were closely related to the members of the genus Nocardioides. Two isolates were most closely related to Nocardioides alpinus Cr7-14T (98.05% and 98.19%). The G + C content of the genomic DNA of strains SYSU D00514T and SYSU D00778 were both 72.8%. Strain SYSU D00514T and the type strains of the genus Nocardioides in the analysis had average nucleotide identity values of 76.8-85.8% as well as digital DNA-DNA hybridization values between 19.5% and 28.0%. The predominant cellular fatty acids of strain SYSU D00514T were C17:1 ω8c, C18:1 ω9c and iso-C16:0. For strain SYSU D00514T, the major polar lipids encompassed diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, an unidentified aminophospholipid and two unidentified aminolipids. The main respiratory quinone of strain SYSU D00514T was MK-8 (H4). Based on the results of polyphasic taxonomic experiments, we propose that strains SYSU D00514T and SYSU D00778 represent a novel species of the genus Nocardioides, with the name Nocardioides xinjiangensis. The type strain is SYSU D00514T (= CGMCC 1.18622T = MCCC 1K05001T = KCTC 49488T).