Nitrite effect on ammonium and nitrite oxidizing processes in a nitrifying sludge

Abstract

Nitrite accumulation can be undesirable in nitrifying reactors used for the biological elimination of nitrogen from wastewaters because the ammonium oxidation process was seen to be inhibited. There is a need to better understand the effects of nitrite on both ammonium and nitrite oxidizing processes. In this paper, the effect of nitrite on the nitrifying activity of a sludge produced in steady-state nitrification was evaluated in batch cultures. At 25 mg N/l of added nitrite, nitrification was successfully carried out. Addition of higher nitrite concentrations to nitrifying cultures (100 and 200 mg N/l) provoked inhibitory effects on the nitrification respiratory process. Nitrite at 100 and 200 mg N/l induced a significant decrease in the values for nitrate yield (−20% and −34%, respectively) and specific rate of nitrate formation (−26% and −67%, respectively), while the ammonium consumption efficiency kept high and the specific rate of ammonium oxidation did not significantly change. This showed that the nitrite oxidizing process was more sensitive to the presence of nitrite than the ammonium oxidizing process. These results showed that as a consequence of nitrite accumulation in nitrification systems, the activity of the nitrite oxidizing bacteria could be more inhibited than that of the ammonium oxidizing bacteria, provoking a higher accumulation of nitrite in the medium.