Abstract
The nitrile hydratase activity of Nocardia corallina B-276 is described, and the only detected product during the biocatalyzed hydrolysis of selected nitriles was the corresponding amide. The best results were obtained using a suspension of cells in a phosphate buffer (pH= 7.0), with conversion percentages ranging from 31% to > 99%.
Highlights
Nitrile is a functional group that usually is transformed to amides or carboxylic acids under strong reaction conditions in acidic or basic media and high temperatures
In Japan acrylamide is produced by the hydrolysis of acrylonitrile using resting cells of Rhodococcus rhodochrous J1;4, 5 and 2-arylpropionitriles have been enantioselectively hydrolyzed to the corresponding amides or carboxylic acids using the bacteria of the genera Rhodococcus,[6,7,8,9] Agrobacterium,[9,10] and Pseudomonas.[11]
In method A we use the cells in the culture media and in method B we use the resting cells suspended in a phosphate buffer
Summary
Nitrile is a functional group that usually is transformed to amides or carboxylic acids under strong reaction conditions in acidic or basic media and high temperatures. To determine the possible hydrolysis of benzamide (1b) to benzoic acid under the conditions of method B, the reaction was performed with a ratio substrate:cells of 1:40, and after 48 h the corresponding carboxylic acid was not detected by HPLC, and the amide 1b remained unchanged. In this case the results of the biocatalyzed hydrolysis under the reaction conditions of method B were much better.
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