Abstract
Nitric oxide (NO) is an endogenously produced molecule that has been implicated in several wound healing mechanisms. Its topical delivery may improve healing in acute or chronic wounds. In this study an antimicrobial peptide was synthesized which self-assembled upon a pH shift, forming a hydrogel. The peptide was chemically functionalized to incorporate a NO-donor moiety on lysine residues. The extent of the reaction was measured by ninhydrin assay and the NO release rate was quantified via the Griess reaction method. The resulting compound was evaluated for its antimicrobial activity against Escherichia coli, and its effect on collagen production by fibroblasts was assessed. Time-kill curves point to an initial increase in bactericidal activity of the functionalized peptide, and collagen production by human dermal fibroblasts when incubated with the NO-functionalized peptide showed a dose-dependent increase in the presence of the NO donor within a range of 0–20 μM.
Highlights
Nitric oxide (NO) is an important biosignalling molecule with regulatory functions in the cardiovascular, immune, and central and peripheral nervous systems
Results of PicoGreen assays are shown in orange in Figure and indicate that NO
These results are consistent within microscopic observations, in which some produces no significantly negative outcomes on cell number up to a concentration of μM, cell detachment could be observed for concentrations above 50 μM
Summary
Nitric oxide (NO) is an important biosignalling molecule with regulatory functions in the cardiovascular, immune, and central and peripheral nervous systems. Different studies have suggested that nitric oxide synthesis is correlated to successful outcomes of wound healing. Endothelial NOS wound closure in iNOS knockout mice when compared with wildtype animals [3]. Endothelial NOS plays a critical role in wound healing mechanisms. A study of excisional wound repair in eNOS plays a critical role in wound healing mechanisms. A study of excisional wound repair in eNOS knockout mice was observed to result in delayed wound closure time when compared with wildtype knockout mice was observed to result in delayed wound closure time when compared with wildtype controls, as well as decreased incisional wound tensile strength [4]. It is reasonable to expect that controls, as well as decreased incisional wound tensile strength [4].
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