Abstract
Trace amounts of nitric oxide (NO) have been determined in aqueous phosphate buffersolutions (pH=7.4) by using a glassy carbon electrode coated with three charge-different polymerfilms. The glassy carbon electrode was coated first with negatively charged Nafion film containingtetrakis(pentafluorophenylporphyrin) iron(III) chloride (Fe(III)TPFPP) as the NO oxidation catalyst,and then with positively charged poly(acrylamide-co-diallyldimethylammonium chloride) (PADDA)and with neutral poly(dimethylsiloxane) (silicone) at the outermost layer. This polymer-coatedelectrode showed an excellent selectivity towards NO against possible concomitants in blood such asnitrite, ascorbic acid, uric acid, and dopamine. All current ratios between each concomitant and NOat the cyclic voltammogram was in 10-3 ~ 10-4. This type of electrode showed a detection limit of80 nM for NO. It was speculated from the electrochemical study in methanol that high-valent oxoiron(IV) of Fe(TPFPP) participated in the catalytic oxidation of NO.
Highlights
Nitric oxide (NO) plays significant roles in the microbiology, physiology and chemistry of an environment
Since Malinski and Taha [12] first clarified that electropolymerized Ni(II)porphyrin was effective for the high-sensitive detection of nitric oxide, many metalloporphyrins [13,14,15,16,17,18,19,20] had been investigated to clarify their functions in the catalysis
Present authors studied on various iron porphyrins [18] and found that tetrakis(pentafluorophenylporphyrin) iron(III) chloride (Fe(TPFPP)Cl) was an excellent catalyst for nitric oxide oxidation
Summary
Nitric oxide (NO) plays significant roles in the microbiology, physiology and chemistry of an environment. Present authors studied on various iron porphyrins [18] and found that tetrakis(pentafluorophenylporphyrin) iron(III) chloride (Fe(TPFPP)Cl) was an excellent catalyst for nitric oxide oxidation We utilized this porphyrin as the oxidative detection of nitric oxide in this work. As the polymer for electrode coating, negatively charged Nafion alcoholic solution (Aldrich, 5 wt%), positively charged poly(acrylamide-co-diallyldimethylammonium chloride) (PADDA) aqueous solution (Aldrich, 10 wt%), and neutral poly(dimethylsiloxane) in xylene (Toray-Dow, KE-9140, 60 wt%) were used. The latter two solutions were diluted to 5 wt% and 10 wt% with the original solvent, respectively. This gas was passed for 30 min before passing NO
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