Abstract
Gramine was extracted from fresh and freeze-dried cereal leeaf tissue according to a simplified methanol/ammonia extraction procedure and quantified by reversed-phase HPLC without further sample preparation. Chromatography was achieved using a modified Pico-Tag solvent system with gradient elution. Quantitative recovery (76-78%) of gramine (17-871 μg/mL) from plant samples was achieved. Recovery of 3-(aminomethyl)indole (AMI), N-methyl-3-(aminomethyl)indole (MAMI), and tryptamine, intermediates in the biosynthesis of gramine, was also demonstrated
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