Abstract
Ionic liquids (ILs) with buffering and chelating abilities were designed and synthesized on the basis of ethylenediaminetetraacetic acid (EDTA) for the development of buffered enzymatic IL systems and for enzymatic reaction in heavy metal containing aqueous system. Transesterification activity of Candida antarctica lipase B dissolved in the hydroxyl-functionalized IL was buffer dependent. High activity and outstanding stability was obtained with the buffered enzymatic IL systems for the transesterification. In heavy metal containing aqueous system, EDTA IL buffers as Hg2+ chelators protected horseradish peroxidase (HRP) against Hg2+-induced denaturation and precipitation. Higher pH favored the protection, while at lower pH the protection diminished. We can conclude that the new ILs possess both buffering and chelating abilities.
Highlights
Enzymes catalyze a wide variety of reactions best in heavy-metal-free aqueous environments and at physiological pH with exquisite selectivity and stereospecificity [1]
The titration profile of ethylenediaminetetraacetic acid (EDTA) with [BMIM]OH in water expressed 3 buffering-like regions, which are centered at pH 3.90, 6.50 and 8.50, respectively (Figure 1)
The structure of EDTA Ionic liquids (ILs) buffers were shown in Scheme 1
Summary
Enzymes catalyze a wide variety of reactions best in heavy-metal-free aqueous environments and at physiological pH with exquisite selectivity and stereospecificity [1]. An appropriate buffer is needed to closely regulate the ionization state of ionizable groups of the enzyme in both aqueous and non-aqueous media. We have synthesized a new class of ionic liquids (ILs) with buffering behaviour that are referred to as IL buffers, which can be used for control of ionization state of enzymes in non-aqueous media [2].
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