Abstract
Serious pine needle disease, Dothistroma needle blight (DNB), caused by Dothistroma septosporum and D. pini was detected in Ukraine in the period 2004–2005. The aim of this study was to identify the Dothistroma species present on new hosts in Ukraine using different molecular techniques to increase our understanding of the local distribution of these pathogens. The occurrence and distribution of DNB were studied between the years 2014 and 2018, and 480 needle samples were collected from 16 different regions in 96 localities—the presence of DNB was confirmed in 62 of them. The host range of DNB consisted of eight pine species, including three subspecies and two spruce species, among them Pinus nigra subsp. pallasiana and P. sylvestris were the most frequent hosts. Results showed that both D. septosporum and D. pini were present on P. nigra subsp. pallasiana on the same trees and even in the same needles. Moreover, D. septosporum was found first in Ukraine on Pinus ponderosa Douglas, Pinus banksiana Lamb and Pinus contorta Douglas in the arboretum as well as Picea pungens Engelm and Picea abies (L.) H. Karst. The suitability for the disease in the different forest types and the intensity of the disease are discussed.
Highlights
Dothistroma needle blight (DNB) has been reported as one of the most serious diseases of conifers with a worldwide distribution and over 100 reported pine host species [1]
Ukrainian isolates from P. nigra subsp. pallasiana were identified as D. pini based on both the internal transcribed spacer (ITS) and the -tubulin sequences [3], presenting the first verified report of this fungus outside North-Central USA
The occurrence and distribution of DNB were studied in the years 2014–2018, and 480 needle samples were collected from 16 different regions in 96 localities—the presence of DNB was confirmed in 62 of them
Summary
Dothistroma needle blight (DNB) has been reported as one of the most serious diseases of conifers with a worldwide distribution and over 100 reported pine host species [1]. Kaznovsky on Pinus sylvestris L. needles nearby Smela (Kiev province, 25 March 1914; LE 116244, duplicate-CBS 11381) [2]. This species was identified as Dothistroma septosporum. Second findings in Ukraine in Mykolaiv and Kherson regions were collected in 2004; DNB was found on Pinus nigra subsp. Pallasiana, (Crimean pine) in Ukraine [2] and this species was identified as D. pini [3]. Two new exotic pine hosts for D. septosporum, Pinus nigra var. Two new exotic pine hosts for D. septosporum, Pinus nigra var. australica and P. nigra var. mollet were recorded in western Ukraine [6]
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