Neural responses from the wind-sensitive interneuron population in four cockroach species

Abstract

The wind-sensitive insect cercal sensory system is involved in important behaviors including predator detection and initiating terrestrial escape responses as well as flight maintenance. However, not all insects possessing a cercal system exhibit these behaviors. In cockroaches, wind evokes strong terrestrial escape responses in Periplaneta americana and Blattella germanica, but only weak escape responses in Blaberus craniifer and no escape responses in Gromphadorhina portentosa. Both P. americana and B. craniifer possesses pink flight muscles correlated with flight ability while B. germanica possesses white flight muscles that cannot support flight and G. portentosa lacks wings. These different behavioral combinations could correlate with differences in sensory processing of wind information by the cercal system. In this study, we focused on the wind-sensitive interneurons (WSIs) since they provide input to the premotor/motor neurons that influence terrestrial escape and flight behavior. Using extracellular recordings, we characterized the responses from the WSI population by generating stimulus–response (S–R) curves and examining spike firing rates. Using cluster analysis, we also examined the activity of individual units (four per species, though not necessarily homologous) comprising the population response in each species. Our main results were: (1) all four species possessed ascending WSIs in the abdominal connectives; (2) wind elicited the weakest WSI responses (lowest spike counts and spike rates) in G. portentosa; (3) wind elicited WSI responses in B. craniifer that were greater than P. americana or B. germanica; (4) the activity of four individual units comprising the WSI population response in each species was similar across species.