Abstract
In this study, we report the nearly complete genome sequences of one enterovirus 96 (EV-C96) isolate (strain 3499/BRA-PA/2010) and two enterovirus 99 (EV-C99) isolates (strains 3291/BRA-PA/2010 and 3944/BRA-PA/2011). The genetic characterization of different enterovirus strains allows for a better understanding of their molecular epidemiology and viral evolution.
Highlights
Enteroviruses are members of the Enterovirus genus and Picornaviridae family
The cDNA was quantified using the Qubit double-stranded DNA high-sensitivity (HS) assay kit (Thermo Fisher Scientific), and the integrity was checked with the 2100 Bioanalyzer instrument (Agilent)
The construction of the cDNA libraries was performed in two steps, namely, fragmentation of total RNA followed by synthesis of double-stranded cDNA, using a cDNA synthesis system kit (Roche) according to the manufacturer’s instructions
Summary
Enteroviruses are members of the Enterovirus genus and Picornaviridae family. These nonenveloped single-stranded RNA viruses have a genome of 7.5 kb and can infect both humans and animals. Total RNA was extracted from 400 l of the cell culture supernatant from each sample. We used the automated RNA extraction and purification protocol from the iPrep PureLink virus kit (Thermo Fisher Scientific).
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