Abstract
AbstractSoybean isoflavones are of considerable interest in relation to their possible health effects in human diets. The rapid and economical determination of soybean isoflavone concentrations is essential for the investigation and development of soybean health foods as well as the selection of soybean seeds with optimal isoflavone levels for such foods. Fourier transforms near infrared reflectance spectroscopy (FT-NIRS) calibrations were developed for the rapid and cost-effective analysis of isoflavones in soybean seeds. FT-NIRS measurements were carried out in quadruplicate for 50 soybean lines selected from the USDA Soybean Germplasm Collection. The selected soybean seeds provided a wide range of isoflavone concentrations (from 0.3 to 6.0 mg/g) that is necessary for development of high-quality calibrations. Laboratory reference values of isoflavone composition were obtained by HPLC analysis of extracted soybean powders. Single soybean seeds were selected for each standard sample and were cut in half in order to avoid screening of the isoflavones NIR absorption bands by the seed coat. For comparison purposes, measurements were also made on soybean powders of the same samples. FT -NIR spectra were collected with a spectral range from 4000 to 12000 cm-1 at a resolution of 8 cm-1 on a Perkin-Elmer Spectrum one NTS spectrometer model. This spectrometer is optimized for high sensitivity analysis of single seed composition, being equipped with an NIRA, integrating sphere accessory and an extended range InGaAs detector.
Highlights
Soybean isoflavones are of considerable interest in relation to their possible health effects in human diets
FT-NIR spectra of half soybean seeds were pre processed before applying a suitable Multiplicative Scattering Correction (MSC)
Our isoflavone calibrations are characterized by low standard errors and high degrees of correlation
Summary
1. University of Illinois at Urbana, AFC- NMR and NIR Microspectroscopy Facility, College of ACES, Urbana, IL. 2. AFC-NMR & NIR Facility, College of ACES, University of Illinois at Urbana 3. University of Illinois at Urbana, AFC- NMR and NIR Microspectroscopy Facility, College of ACES 4. Crop Sciences Department and National Soybean Laboratory, College of ACES, University of Illinois at Urbana 5. NPRE Department, University of Illinois at Urbana
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