Abstract
Various bioactive agrowastes derived phenolic compounds (APCs) obtained from agrowastes lignin hydrolyzed methods and evaluated for their potential antioxidant, antimicrobial and anti-quorum activities with special reference to food borne pathogens. Half-maximal inhibitory concentrations (IC50) of isolated phenolic compounds against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals have been found in the range of 53–84 µg/mL, whereas samples obtained by hydrolysis in absence of acid have shown the (IC50) values of ~ 120 µg/mL. The APCs have shown significant antimicrobial activity against Gram positive (Bacillus subtilis) as compared to Gram negative (Pseudomonas aeruginosa) bacteria. The bacterial killing as well as anti-quorum sensing activity (inhibiting the violacein production) has been observed against Chromobacterium violaceum. Flow cytometric analysis has been also confirmed the cell death occurs in Gram positive and Gram negative bacteria. The present study suggests that APCs derived from agro-wastes can serve as an important source of natural antioxidant and antimicrobial agents. The natural ingredients will be considered as emerging materials for food packaging.
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