Abstract
Broadening of spectral lines is a signature of chemical exchange phenomena on microsecond to millisecond time scales but has deleterious effects on spectral resolution and sensitivity. A multipulse method based on chemical shift scaling that reduces chemical exchange broadening during frequency-encoding periods of liquid-state multidimensional NMR experiments is described. The proposed scheme utilizes low-power radiofrequency pulses, which offer the advantages of short cycle times and minimal sample heating. The method is suitable for biological macromolecules, as relaxation not resulting from chemical exchange is reduced by placing the magnetization along the z axis for part of the evolution trajectory. The resolution and sensitivity enhancement for resonances broadened by chemical exchange is demonstrated on the protein ribonuclease A. The work demonstrates the feasibility of applying coherent averaging techniques, which were originally developed in solid-state NMR spectroscopy, to biological NMR spectroscopy in the liquid state for resolution enhancement and facilitates the detection of resonances that are severely broadened by chemical exchange processes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
