Abstract
Protein termini are generally associated with protein stability. Taking advantage of charge state differences between terminal peptides and internal peptides, we developed a new method termed LysargiNase-assisted Analysis of the Protein N- and C-Terminome (NAPT) for selective separation of protein termini. Altogether, we identified 2458 protein N-termini and 3056 protein C-termini from the HeLa cell line.
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