Abstract
Nanosafety evaluation is necessary not only for nanomaterials research and development but also as a cornerstone for legal regulation. In this study, we proposed a non-invasive protocol for nanosafety evaluation through feces using metagenomics and metabolomics together with metallomics. Male SD rats were orally exposed to equal amounts of Se (Se0NPs, 3.14 or Na2SeO3, 6.28 μg/kg bw) and were sacrificed after 24 h. 16S rRNA analysis and LC–MS were used to study the impact of different forms of Se on intestinal microbiota and metabolites in fecal samples. ICP-MS and synchrotron radiation X-ray fluorescence (SR-XRF) and X-ray absorption spectroscopy (XAS) were used to study the concentration and transformation of Se in gastrointestinal track and feces. It was found that Se0NPs brought less alternation to the composition of intestinal microbiota and metabolites related to inflammation, immunity and gut-brain axis related responses than Na2SeO3 did. Besides, the absorbed Se0NPs could be more efficiently converted to organic Se which explained the comparable bioavailability to Na2SeO3. In all, the proposed protocol combined with metagenomics, metabolomics and metallomics in this study can be used for nanosafety evaluation in a non-invasive manner and this may also be used for the screening of the nanosafety of other nanomaterials.
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