Abstract
In this study, a multiplex detection system was proposed by integrating a localized surface plasmon resonance (LSPR) sensing array and parallel microfluidic channels. The LSPR sensing array was fabricated by nanoimprinting and gold sputter on a polycarbonate (PC) substrate. The polydimethylsiloxane (PDMS) microfluidic channels and PC LSPR sensing array were bound together through (3-aminopropyl)triethoxysilane (APTES) surface treatment and oxygen plasma treatment. The resonant spectrum of the LSPR sensing device was obtained by broadband white-light illumination and polarized wavelength measurements with a spectrometer. The sensitivity of the LSPR sensing device was measured using various ratios of glycerol to water solutions with different refractive indices. Multiplex detection was demonstrated using human immunoglobulin G (IgG), IgA, and IgM. The anti-IgG, anti-IgA, and anti-IgM were separately modified in each sensing region. Various concentrations of human IgG, IgA, and IgM were prepared to prove the concept that the parallel sensing device can be used to detect different targets.
Highlights
Label-free biosensors generally use a transducer to convert biological signals, received from a recognition element, into readable information
We propose a parallel detection method through a nanoplasmonic sensing array integrated with parallel microfluidic channels that expands one major channel into four branching channels through two cascades
The results indicated with increasing cated that the developed nanoplasmonic sensing array integrated in parallel with a miIgG, IgA, and IgM concentrations, the resonant spectra of the nanoplasmonic sensing crofluidic device could be used for multiplex detection
Summary
Label-free biosensors generally use a transducer to convert biological signals, received from a recognition element, into readable information. The transducer is the critical element for determining the sensitivity, sensing range, and detection limit of the biosensors. Several transducers for label-free immunosensing were developed, including quartz crystal microbalances (QCMs) [1,2,3], amperometric transducers [4,5,6,7], cantilever beams [8,9,10], electrochemical impedance spectroscopy (EIS) biosensors [11,12,13,14], preconcentrated immunosensing [15,16,17], surface plasmon resonance (SPR) [18,19,20], and nanobead. Conventional reflective-type SPR platforms utilize a prism-coupled gold thin film on a glass substrate to induce an evanescent wave as the resonant wavelength that can be changed based on the biomolecular binding affinity
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